Ordodi Valentin L, Paunescu Virgil, Mischie Septimiu, Ignea Alimpie, Toma Octavian, Ionac Mihai, Mic Ani A, Sandesc Dorel, Mic Felix A
Department of Physiology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
J Am Assoc Lab Anim Sci. 2006 Nov;45(6):54-7.
Experimental induction of ventricular fibrillation in animals yields valuable information about this deadly arrhythmia. Human adult or pediatric defibrillators and their paddles can be used easily in larger animals such as dogs and pigs, but these animals are more difficult to house and handle, and available biochemical assays may be limited. In contrast, rats are easy and relatively inexpensive to house and handle, and numerous biochemical tests are available. However, in most cases, even pediatric electrodes are impractical for use in rats. Proper placement of defibrillation electrodes on the thorax requires that the electrical axis of the heart be situated between the defibrillator paddles. The most common approach to defibrillation in rats uses 2 electrodes: one is built into a board that underlies and touches the rat's back, and another is positioned manually on the anterior thorax. The aim of this study was to produce electrodes that are 1) easy to handle, 2) specifically designed for rats, 3) efficiently deliver defibrillation shocks along the electric axis of the heart, and 4) can be used for both in vivo defibrillation and on isolated heart preparations.
在动物身上实验性诱发心室颤动可获得有关这种致命性心律失常的宝贵信息。成人或儿童用的除颤器及其电极板可轻松用于如狗和猪等较大型动物，但这些动物饲养和处理起来更困难，且可用的生化检测方法可能有限。相比之下，大鼠饲养和处理起来容易且成本相对较低，并且有大量生化检测方法可用。然而，在大多数情况下，即使是儿童电极在大鼠身上使用也不实用。除颤电极在胸部的正确放置要求心脏电轴位于除颤器电极板之间。大鼠除颤最常用的方法是使用两个电极：一个内置在一块置于大鼠背部下方并与之接触的板中，另一个手动放置在前胸部。本研究的目的是制作出满足以下条件的电极：1）易于操作；2）专门为大鼠设计；3）能沿心脏电轴有效传递除颤电击；4）可用于体内除颤和离体心脏标本。
