未取代罗丹明戊酯对大鼠肝线粒体呼吸及Ca2+转运的影响。

Effects of amyl ester of unsubstituted rhodamine on respiration and Ca2+ transport in rat liver mitochondria.

作者信息

Krasnikov B F, Avad A S, Zorov D B, Yaguzhinsky L S

机构信息

A.N.Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.

出版信息

Biochem Biophys Res Commun. 1991 Mar 29;175(3):1010-6. doi: 10.1016/0006-291x(91)91665-y.

DOI:10.1016/0006-291x(91)91665-y

PMID:1709008

Abstract

In rat liver mitochondria the amylrhodamine is responsible for uncoupling (respiratory stimulation in state 4) by two distinct processes. Immediately after amylrhodamine addition (2-12 microM) stimulation of respiration takes place. Respiration rate for this phase is constant in time, it is independent of the potassium or inorganic phosphate content in the medium, not inhibited by oligomycin, ruthenium red, cyclosporine A, N-ethyl-maleimide and EGTA. The second phase of the respiratory stimulation is not linear in time. Respiration rate within this phase increases with rising of potassium and phosphate content in the medium. This effect is abolished by oligomycin, ruthenium red, cyclosporine A, N-ethylmaleimide and EGTA. The beginning of respiratory increment coincides with the second phase of Ca2+ release from mitochondria.

摘要

在大鼠肝脏线粒体中，淀粉若丹明通过两种不同的过程导致解偶联（状态4下的呼吸刺激）。添加淀粉若丹明（2 - 12微摩尔）后立即发生呼吸刺激。此阶段的呼吸速率随时间恒定，与培养基中的钾或无机磷酸盐含量无关，不受寡霉素、钌红、环孢素A、N - 乙基马来酰亚胺和乙二醇双乙胺醚（EGTA）的抑制。呼吸刺激的第二阶段在时间上不是线性的。此阶段内的呼吸速率随着培养基中钾和磷酸盐含量的增加而增加。寡霉素、钌红、环孢素A、N - 乙基马来酰亚胺和乙二醇双乙胺醚（EGTA）可消除这种效应。呼吸增加的开始与线粒体中钙离子释放的第二阶段同时发生。