Runge-Morris M, Wilusz J
Institute of Chemical Toxicology, Wayne State University, Detroit, MI 48201.
Biochem Biophys Res Commun. 1991 Mar 29;175(3):1051-6. doi: 10.1016/0006-291x(91)91671-x.
Hepatic hydroxysteroid sulfotransferase-a (HST-a) gene expression was examined in young male (age 22-26 days) and female rats (age 22-30 days), and in older male (age 42-45 days) and female (age 49-55 days) rats. Northern and slot blot analyses of poly(A)+RNA revealed that HST-a was differentially expressed with respect to both age and gender with female rats expressing higher levels of HST-a in both age groups. Hepatic HST-a mRNA levels were approximately 4 to 6-fold higher in females compared to males in both age groups examined. HST-a expression increased with age in both male and female rats. HST-a expression was approximately 8 to 10-fold higher in 42-45 day old males relative to 22-26 day old males. HST-a mRNA levels were approximately 3 to 7-fold higher in 49-55 day old females relative to females in the 22-30 day age group. These data suggest that HST-a gene expression is transcriptionally controlled and that HST-a regulation is subject to hormonal and developmental modulation.
在年轻雄性（22 - 26日龄）和雌性大鼠（22 - 30日龄）以及老年雄性（42 - 45日龄）和雌性大鼠（49 - 55日龄）中检测了肝羟类固醇硫酸转移酶-a（HST-a）基因的表达。对多聚腺苷酸（poly(A)+）RNA进行的Northern印迹和狭缝印迹分析显示，HST-a在年龄和性别方面均存在差异表达，在两个年龄组中雌性大鼠表达的HST-a水平更高。在所检测的两个年龄组中，雌性大鼠肝脏中的HST-a mRNA水平比雄性大鼠高约4至6倍。在雄性和雌性大鼠中，HST-a的表达均随年龄增加。42 - 45日龄雄性大鼠的HST-a表达相对于22 - 26日龄雄性大鼠高约8至10倍。49 - 55日龄雌性大鼠的HST-a mRNA水平相对于22 - 30日龄组的雌性大鼠高约3至7倍。这些数据表明，HST-a基因表达受转录控制，且HST-a的调节受到激素和发育的调控。
