Runge-Morris M A
Institute of Chemical Toxicology, Wayne State University, Detroit, MI 48201.
Chem Biol Interact. 1994 Jun;92(1-3):67-76. doi: 10.1016/0009-2797(94)90054-x.
Enzymatic sulfation has been implicated to play a key role in a number of essential biological pathways including xenobiotic detoxication, carcinogen activation, and the regulation of intra-tissue hormone activity. In order to increase our understanding of the critical determinants governing the regulation of sulfotransferase gene expression, we investigated age-, gender-, and xenobiotic-related alterations in hydroxysteroid sulfotransferase-a or aryl sulfotransferase-IV gene expression. Northern blot and slot blot analyses showed that rat hepatic hydroxysteroid sulfotransferase-a mRNA expression was responsive to age- and gender-related signals. The results also suggested that the rat hepatic aryl sulfotransferase-IV and hydroxysteroid sulfotransferase-a genes are differentially regulated. Northern blot and reverse transcriptase polymerase chain reaction analyses demonstrated that hydroxysteroid sulfotransferase-a mRNA was expressed to a greater extent in female rat liver than in lung or kidney tissue. In addition, rat hepatic hydroxysteroid sulfotransferase-a gene expression in mature female rats, although not substantially altered in response to short-term fasting or high-dose dexamethasone treatment, was suppressed after treatment with the polycyclic aromatic hydrocarbon, 3-methylcholanthrene.
酶促硫酸化作用在许多重要的生物学途径中发挥关键作用，这些途径包括外源性物质解毒、致癌物激活以及组织内激素活性的调节。为了增进我们对控制硫酸转移酶基因表达调控的关键决定因素的理解，我们研究了羟类固醇硫酸转移酶-a或芳基硫酸转移酶-IV基因表达中与年龄、性别和外源性物质相关的变化。Northern印迹和狭缝印迹分析表明，大鼠肝脏羟类固醇硫酸转移酶-a mRNA表达对与年龄和性别相关的信号有反应。结果还表明，大鼠肝脏芳基硫酸转移酶-IV和羟类固醇硫酸转移酶-a基因受到不同的调控。Northern印迹和逆转录聚合酶链反应分析表明，羟类固醇硫酸转移酶-a mRNA在雌性大鼠肝脏中的表达程度高于肺或肾组织。此外，成熟雌性大鼠肝脏中的羟类固醇硫酸转移酶-a基因表达，虽然在短期禁食或高剂量地塞米松处理后没有明显改变，但在用多环芳烃3-甲基胆蒽处理后受到抑制。
