Zeng Deyi, Shi Haiyan, Li Bo, Wang Minghua, Song Baoan
Department of Pesticide, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
J Agric Food Chem. 2006 Nov 15;54(23):8682-7. doi: 10.1021/jf061492n.
Accurate quantification of quizalofop-p-ethyl is essential for it may do harm to humans and animals through both water and food. Currently, detection of quizalofop-p-ethyl mainly relies on methods such as gas chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry. Although these techniques are reliable, they are relatively expensive and time-consuming because of multistep sample cleanup. To address this, we developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) with a polyclonal antibody against quizalofop-p-ethyl that was generated in our lab. The IC(50) of detection was 0.03495 microg/mL, and the lowest detection limit reached 0.00192 microg/mL. Furthermore, the method had high specificity for it did not cross-react with other structure-related compounds. When water and soil samples that were fortified with quizalofop-p-ethyl were analyzed by this ELISA, recoveries were in the range of 89-110% from water and 81-108% from soil. Good correlations between this immunoassay and gas chromatography data were obtained for residues of quizalofop-p-ethyl in water and soil. Our data indicate that this method is a convenient analytical technique for monitoring quizalofop-p-ethyl in waters without extraction and the extra cleanup step and in soil without the cleanup step.
精喹禾灵乙酯的准确定量至关重要，因为它可能通过水和食物对人类及动物造成危害。目前，精喹禾灵乙酯的检测主要依赖气相色谱法、高效液相色谱法以及气相色谱 - 质谱联用法等。尽管这些技术可靠，但由于样品需要多步净化处理，所以相对昂贵且耗时。为解决这一问题，我们利用在本实验室制备的针对精喹禾灵乙酯的多克隆抗体制备了一种竞争性间接酶联免疫吸附测定法（ciELISA）。该检测方法的半数抑制浓度（IC50）为0.03495微克/毫升，最低检测限达0.00192微克/毫升。此外，该方法具有高特异性，因为它与其他结构相关化合物无交叉反应。当用此酶联免疫吸附测定法分析添加了精喹禾灵乙酯的水和土壤样品时，水中回收率在89% - 110%之间，土壤中回收率在81% - 108%之间。对于水和土壤中精喹禾灵乙酯的残留量，该免疫测定法与气相色谱数据之间具有良好的相关性。我们的数据表明，该方法是一种便捷的分析技术，可用于监测未经萃取和额外净化步骤的水中以及未经净化步骤的土壤中的精喹禾灵乙酯。
