Carasek Eduardo, Pawliszyn Janusz
Department of Chemistry, University of Waterloo Waterloo, Ontario N2L 3G1, Canada.
J Agric Food Chem. 2006 Nov 15;54(23):8688-96. doi: 10.1021/jf0613942.
In this study, the optimization and comparison of an internally cooled fiber [cold fiber with polydimethylsiloxane (PDMS) loading] and several commercial solid-phase microextraction (SPME) fibers for the extraction of volatile compounds from tropical fruits were performed. Automated headspace solid-phase microextraction (HS-SPME) using commercial fibers and an internally cooled SPME fiber device coupled to gas chromatography-mass spectrometry (GC-MS) was used to identify the volatile compounds of five tropical fruits. Pulps of yellow passion fruit (Passiflora edulis), cashew (Anacardium occidentale), tamarind (Tamarindus indica L.), acerola (Malphigia glabra L.), and guava (Psidium guajava L.) were sampled. The extraction conditions were optimized using two experimental designs (full factorial design and Doehlert matrix) to analyze the main and secondary effects. The volatile compounds tentatively identified included alcohols, esters, carbonyl compounds, and terpernes. It was found that the cold fiber was the most appropriate fiber for the purpose of extracting volatile compounds from the five fruit pulps studied.
在本研究中，对一种内部冷却纤维（填充聚二甲基硅氧烷（PDMS）的冷纤维）和几种商用固相微萃取（SPME）纤维进行了优化和比较，以从热带水果中提取挥发性化合物。使用商用纤维的自动顶空固相微萃取（HS-SPME）和与气相色谱-质谱联用（GC-MS）的内部冷却SPME纤维装置来鉴定五种热带水果的挥发性化合物。采集了黄百香果（西番莲）、腰果（腰果）、罗望子（罗望子）、针叶樱桃（针叶樱桃）和番石榴（番石榴）的果肉。采用两种实验设计（全因子设计和Doehlert矩阵）优化萃取条件，以分析主要和次要影响。初步鉴定出的挥发性化合物包括醇类、酯类、羰基化合物和萜类。结果发现，对于从所研究的五种水果果肉中提取挥发性化合物而言，冷纤维是最合适的纤维。
