Kiss Judit, Korbász Margit, Sass-Kiss Agnes
Department of Analytics, Central Food Research Institute, Herman Ottó út 15, 1022 Budapest, Hungary.
J Agric Food Chem. 2006 Nov 15;54(23):8909-18. doi: 10.1021/jf061578g.
Aliphatic primary and biogenic amines of grape varieties from two vintages were studied. We established that appearance and/or increase of both primary aliphatic and biogenic amines is due to microbiota living in/on grape berries. Aszú, gray rotten grapes infected mainly with Botrytis cinerea, grape berries infected mainly with Penicillium species, and intact grape berries were compared on the basis of amine composition using t-test, analysis of variance, and multivariate statistical analysis (principal component analysis and linear discriminant analysis). The amine composition and amine content of Aszú grapes were significantly different (p < 0.05) from those of intact grapes despite the effect of the vineyards and the vintages. Grape samples coming from the same growing location, intact, Aszú grapes, and grape berries infected mainly with Penicillium species could be separated from each other with multivariate statistical analysis. We distinguished intact, Aszú (noble rotten), and gray rotten berries from each other as well. Evaluating amine values of grape samples independently of the place of origin, the Aszú and green rotten berries could not be differentiated. The varieties and vineyards have affected the amine composition of Aszú grapes, while these effects on intact grapes appeared only slightly.
对两个年份的葡萄品种中的脂肪族伯胺和生物胺进行了研究。我们确定，脂肪族伯胺和生物胺的出现和/或增加是由于生活在葡萄浆果内部和/或表面的微生物群所致。使用t检验、方差分析和多元统计分析（主成分分析和线性判别分析），对主要感染灰霉病的阿苏（Aszú）葡萄、主要感染青霉属的灰腐葡萄以及完整葡萄浆果的胺组成进行了比较。尽管受到葡萄园和年份的影响，但阿苏葡萄的胺组成和胺含量与完整葡萄相比仍存在显著差异（p < 0.05）。来自相同种植地点的完整葡萄、阿苏葡萄以及主要感染青霉属的葡萄浆果样本可以通过多元统计分析相互区分。我们也能够区分完整浆果、阿苏（贵腐）浆果和灰腐浆果。在不考虑葡萄样本产地的情况下评估胺值时，无法区分阿苏葡萄和绿腐葡萄。品种和葡萄园对阿苏葡萄的胺组成产生了影响，而对完整葡萄的影响则较为轻微。
