González-Cámpora R, Armas-Padrón J R, Rios-Martín J J, López-Garrido J, Gómez-Pascual A, Galera-Davidson H
Department of Pathology, University of Seville, Spain.
Anal Quant Cytol Histol. 1991 Feb;13(1):16-22.
Forty-one cases of typical melanocytic skin lesions (15 intradermal nevi, 14 Spitz nevi and 12 malignant melanomas) were used to investigate the value of staining of nucleolar organizer regions (NORs) in the differential diagnosis of such pigmented lesions. Histologic sections were stained by the silver colloid (Ag) method, with and without the prior use of a melanin blocking agent. There were statistically significant differences in the mean numbers of AgNORs per nucleus between the groups of lesions studied (1.658 for intradermal nevi, 3.0042 for Spitz nevi and 6.669 for malignant melanomas). Sections treated with potassium permanganate (melanin blocking agent) prior to staining showed an obvious increase in the AgNOR scores in all groups; this increase was highest for Spitz nevi. Although AgNOR staining allows a distinction to be made between intradermal nevi and malignant melanomas, the striking overlap between the counts for Spitz nevi and malignant melanomas precludes the use of this technique as the sole method for establishing the diagnosis of malignancy. Other clinical and morphologic data are especially required to make the diagnosis of Spitz nevi.
41例典型黑素细胞性皮肤病变（15例皮内痣、14例Spitz痣和12例恶性黑素瘤）用于研究核仁组成区（NORs）染色在这类色素性病变鉴别诊断中的价值。组织学切片采用银胶体（Ag）染色法，染色前分别使用和不使用黑色素阻断剂。在所研究的病变组之间，每个细胞核的平均AgNORs数量存在统计学显著差异（皮内痣为1.658，Spitz痣为3.0042，恶性黑素瘤为6.669）。染色前用高锰酸钾（黑色素阻断剂）处理的切片在所有组中均显示AgNOR评分明显增加；Spitz痣的增加最为显著。虽然AgNOR染色可区分皮内痣和恶性黑素瘤，但Spitz痣和恶性黑素瘤计数之间的显著重叠使得不能将该技术作为确立恶性诊断的唯一方法。诊断Spitz痣尤其需要其他临床和形态学数据。
