Ishii Shun'ichi, Unno Hajime, Miyata Shunsuke, Hori Katsutoshi
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama, Japan.
Biosci Biotechnol Biochem. 2006 Nov;70(11):2635-40. doi: 10.1271/bbb.60236. Epub 2006 Nov 7.
A toluene-degrading bacterium, Acinetobacter sp. Tol 5, shows noteworthy adhesiveness mediated by two types of cell appendages. In this study, we obtained a less-adhesive mutant, T1, which lost both types of appendages, and investigated how the cell appendages affect the adhesion properties of this useful bacterium for environmental technology. Wild-type cells attained irreversible adhesion to polyurethane carriers within 30 s, while adhesion of T1 cells was still reversible at that time. While T1 showed decreased adhesion with decreasing ionic strength and did not adhere at all at 0.015 mM, adhesion of the wild type was fully independent of ionic strength. Acinetobacter sp. Tol 5 was also found to be not motile. Our results suggest that through the long distant interaction mediated by the appendages between the cells and surfaces, Tol 5 cells can attain irreversible adhesion very quickly without approaching the vicinity of the substratum.
一种降解甲苯的细菌，不动杆菌属Tol 5菌株，表现出由两种细胞附属物介导的显著粘附性。在本研究中，我们获得了一种粘附性较低的突变体T1，它失去了这两种附属物，并研究了细胞附属物如何影响这种对环境技术有用的细菌的粘附特性。野生型细胞在30秒内对聚氨酯载体实现了不可逆粘附，而此时T1细胞的粘附仍然是可逆的。虽然T1的粘附随着离子强度的降低而降低，在0.015 mM时完全不粘附，但野生型的粘附完全不受离子强度的影响。还发现不动杆菌属Tol 5菌株不具有运动性。我们的结果表明，通过附属物介导的细胞与表面之间的长距离相互作用，Tol 5细胞可以在不靠近基质附近的情况下非常迅速地实现不可逆粘附。
