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通过泛素连接酶Rsp5和泛素结合酶的过表达增强酿酒酵母的胁迫耐受性。

Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymes.

作者信息

Hiraishi Hiroyuki, Mochizuki Mai, Takagi Hiroshi

机构信息

Bio-Oriented Technology Research Advancement Institution (BRAIN), Japan.

出版信息

Biosci Biotechnol Biochem. 2006 Nov;70(11):2762-5. doi: 10.1271/bbb.60250. Epub 2006 Nov 7.

DOI:10.1271/bbb.60250
PMID:17090950
Abstract

Yeast Saccharomyces cerevisiae cells overexpressing essential ubiquitin ligase Rsp5 or ubiquitin-conjugating enzymes (Ubc1-Ubc13) showed tolerance to various stresses. Co-overexpression of Rsp5 and Ubc1, Ubc2, Ubc3, Ubc5, Ubc6, Ubc9, Ubc10, Ubc11, Ubc12, or Ubc13 further enhanced stress tolerance. These results suggest that overexpression of ubiquitin-related enzymes might be a useful method for breeding novel stress-resistant strains.

摘要

过表达必需泛素连接酶Rsp5或泛素结合酶（Ubc1-Ubc13）的酿酒酵母细胞表现出对各种胁迫的耐受性。Rsp5与Ubc1、Ubc2、Ubc3、Ubc5、Ubc6、Ubc9、Ubc10、Ubc11、Ubc12或Ubc13的共过表达进一步增强了胁迫耐受性。这些结果表明，泛素相关酶的过表达可能是培育新型抗逆菌株的一种有用方法。

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