Clarke C J, Wilson A D, Williams N A, Stokes C R
Department of Veterinary Medicine, University of Bristol School of Veterinary Science, Langford.
Immunology. 1991 Mar;72(3):323-8.
Cholera toxin is widely recognized as a potent stimulator of mucosal IgA responses after oral feeding. However, comparatively little is known of its ability to stimulate cellular responses. This is due in part to the direct inhibitory effects of cholera toxin on T lymphocytes, which can confound attempts to study primed T-cell responses in vitro. We have avoided this problem by using cholera toxin as an adjuvant to enhance the response to an unrelated protein fed simultaneously. Thus the simultaneous feeding of 10 micrograms of cholera toxin and 5 mg of keyhole limpet haemocyanin (KLH) results in the priming of T cells in the spleen, mesenteric lymph nodes, Peyer's patch and lamina propria that proliferate when restimulated with KLH in vitro. The feeding of KLH alone does not result in such responses. Both CD4- and CD8-positive antigen-specific T cells were involved in the response and the cells produced both interleukins 2 and 4 on antigen restimulation in vitro.
霍乱毒素被广泛认为是口服后黏膜IgA反应的有效刺激物。然而，对于其刺激细胞反应的能力，人们了解得相对较少。部分原因在于霍乱毒素对T淋巴细胞具有直接抑制作用，这可能会干扰体外研究致敏T细胞反应的尝试。我们通过使用霍乱毒素作为佐剂来增强对同时投喂的无关蛋白质的反应，从而避免了这个问题。因此，同时投喂10微克霍乱毒素和5毫克钥孔戚血蓝蛋白（KLH）会导致脾脏、肠系膜淋巴结、派尔集合淋巴结和固有层中的T细胞致敏，这些T细胞在体外再次用KLH刺激时会增殖。单独投喂KLH不会产生这种反应。CD4阳性和CD8阳性的抗原特异性T细胞都参与了反应，并且这些细胞在体外抗原再刺激时会产生白细胞介素2和4。
