Hao Jianrong, Ma Cuiqing, Gao Chao, Qiu Jianhua, Wang Min, Zhang Yinan, Cui Xing, Xu Ping
State Key Laboratory of Microbial Technology, Shandong University, Jinan, PR China.
Biotechnol Lett. 2007 Jan;29(1):105-10. doi: 10.1007/s10529-006-9204-6. Epub 2006 Nov 8.
Pseudomonas stutzeri SDM oxidized DL-lactic acid (25.5 g l(-1)) into pyruvic acid (22.6 g l(-1)) over 24 h. Both NAD(+)-independent D-lactate dehydrogenase and NAD(+)-independent L-lactate dehydrogenase were found for the first time in the bioconversion of lactate to pyruvate based on the enzyme activity assay and proteomic analysis.
施氏假单胞菌SDM在24小时内将DL-乳酸（25.5 g l⁻¹）氧化为丙酮酸（22.6 g l⁻¹）。基于酶活性测定和蛋白质组学分析，在乳酸向丙酮酸的生物转化过程中首次发现了不依赖NAD⁺的D-乳酸脱氢酶和不依赖NAD⁺的L-乳酸脱氢酶。
