State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Bioresour Technol. 2012 Apr;110:719-22. doi: 10.1016/j.biortech.2012.01.123. Epub 2012 Jan 30.
2-Oxobutyrate (2-OBA) is an important intermediate with many applications in the drug and chemical industries. l-Threonine, an industrial fermentation product, could be used as a suitable starting material for the 2-OBA production. In this study, whole cells of Pseudomonas stutzeri SDM were confirmed to possess a good ability to convert l-threonine into 2-OBA. The bioconversion conditions were optimized for the 2-OBA production from l-threonine. Using 9.2g dry cell weight l(-1) of whole cells of strain SDM as biocatalyst, the biocatalytic process produced 2-OBA at a high concentration (25.6gl(-1)) with a high molar conversion rate (99.6%) at 6h from 30g1(-1) of l-threonine.
2-氧代丁酸（2-OBA）是一种重要的中间产物，在药物和化学工业中有许多应用。l-苏氨酸是一种工业发酵产物，可以作为 2-OBA 生产的合适起始原料。在本研究中，证实了恶臭假单胞菌 SDM 的完整细胞具有将 l-苏氨酸转化为 2-OBA 的良好能力。优化了 l-苏氨酸生物转化生产 2-OBA 的条件。使用 9.2g 干细胞重量 l(-1)的 SDM 菌株全细胞作为生物催化剂，在 6 小时内从 30g1(-1)的 l-苏氨酸中以高浓度（25.6gl(-1))和高摩尔转化率（99.6%）生产 2-OBA。
