Spoof Lisa, Berg Katri A, Rapala Jarkko, Lahti Kirsti, Lepistö Liisa, Metcalf James S, Codd Geoffrey A, Meriluoto Jussi
Department of Biochemistry and Pharmacy, Abo Akademi University, Tykistökatu 6A, FI-20520 Turku, Finland.
Environ Toxicol. 2006 Dec;21(6):552-60. doi: 10.1002/tox.20216.
The cyanobacterial cytotoxin cylindrospermopsin has been mostly associated with cyanobacteria present in tropical and subtropical regions. Cylindrospermopsin has recently been found in cyanobacterial samples in central and southern Europe but the possible presence of the toxin in northern Europe has been unknown. Fifty-eight field and laboratory culture samples of Finnish cyanobacteria were analyzed by high-performance liquid chromatography combined with UV diode-array detection, multiple reactant monitoring in a triple-quadrupole mass spectrometer (MS), and accurate mass measurements using a time-of-flight MS instrument. Cylindrospermopsin was confirmed by all three techniques in a culture sample of Anabaena lapponica at a concentration of 242 microg cylindrospermopsin per g freeze-dried cyanobacterial material.
蓝藻细胞毒素柱孢藻毒素大多与热带和亚热带地区的蓝藻有关。最近在欧洲中部和南部的蓝藻样本中发现了柱孢藻毒素，但北欧是否可能存在这种毒素一直未知。通过高效液相色谱结合紫外二极管阵列检测、三重四极杆质谱仪中的多反应监测以及使用飞行时间质谱仪进行精确质量测量，对58个芬兰蓝藻的野外和实验室培养样本进行了分析。在一种拉氏鱼腥藻的培养样本中，所有这三种技术都证实了柱孢藻毒素的存在，其浓度为每克冻干蓝藻材料含242微克柱孢藻毒素。
