一种检测胰岛素样生长因子结合蛋白-3（IGFBP-3）蛋白水解作用的简易方法。
A simple assay for proteolysis of IGFBP-3.
作者信息
Lamson G, Giudice L C, Rosenfeld R G
机构信息
Department of Pediatrics, Stanford University Medical Center, California 94305.
出版信息
J Clin Endocrinol Metab. 1991 Jun;72(6):1391-3. doi: 10.1210/jcem-72-6-1391.
An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125IhIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37 degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
已经开发出一种用于评估生物流体或蛋白质对IGFBP - 3进行蛋白水解作用的测定方法，该方法使用重组来源于大肠杆菌的125I - hIGFBP - 3作为底物指示剂。将标记的底物在37摄氏度下于被测试剂存在的情况下进行孵育，通过SDS - PAGE和放射自显影来观察蛋白水解情况。该测定表明，与妊娠相关的蛋白酶依赖于Ca++。该底物也会被大鼠和小鼠的妊娠血清进行蛋白水解，使得该测定方法适用于那些动物系统的研究。
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