促卵泡激素对人黄体化颗粒细胞中胰岛素样生长因子结合蛋白的调控
Regulation of IGF binding proteins by FSH in human luteinizing granulosa cells.
作者信息
Adachi T, Iwashita M, Kuroshima A, Takeda Y
机构信息
Department of Obstetrics and Gynecology, Tokyo Women's Medical College, Japan.
出版信息
J Assist Reprod Genet. 1995 Oct;12(9):639-43. doi: 10.1007/BF02212589.
OBJECTIVE
To evaluate the regulation of the secretion of insulin-like growth factor (IGF) binding proteins (IGFBPs) by FSH in human luteinizing granulosa cells.
DESIGN AND RESULTS
Luteinizing granulosa cells were incubated with and without FSH. Levels of IGFBP-1 and -3 in the medium were measured by EIA and RIA, respectively and binding activities of these IGFBP were evaluated by Western ligand blot. FSH inhibited the secretion of IGFBP-1 dose dependently. FSH did not inhibit the secretion of immunoreactive IGFBP-3, but inhibited the binding activity of IGFBP-3. To assess the protease activity, 125I-IGFBP-3 was incubated with the cultured medium. 125I-IGFBP-3 degraded into small fragments when it was incubated with the cultured medium treated with FSH.
CONCLUSIONS
FSH enhances the action of IGF-I in human granulosa cells by inhibiting the secretion of IGFBP-1 and the binding activity of IGFBP-3 by stimulating the proteolysis of IGFBP-3.
目的
评估促卵泡激素（FSH）对人黄体化颗粒细胞中胰岛素样生长因子（IGF）结合蛋白（IGFBP）分泌的调节作用。
设计与结果
将黄体化颗粒细胞分别在有和无FSH的情况下进行孵育。分别采用酶免疫分析（EIA）和放射免疫分析（RIA）测定培养基中IGFBP-1和IGFBP-3的水平，并通过蛋白质免疫印迹法评估这些IGFBP的结合活性。FSH剂量依赖性地抑制IGFBP-1的分泌。FSH不抑制免疫反应性IGFBP-3的分泌，但抑制IGFBP-3的结合活性。为评估蛋白酶活性，将¹²⁵I-IGFBP-3与培养基一起孵育。当¹²⁵I-IGFBP-3与经FSH处理的培养基一起孵育时，它会降解为小片段。
结论
FSH通过抑制IGFBP-1的分泌以及通过刺激IGFBP-3的蛋白水解来抑制IGFBP-3的结合活性，从而增强IGF-I在人颗粒细胞中的作用。
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