Zharov Vladimir P, Galanzha Ekaterina I, Menyaev Yulian, Tuchin Valery V
Philips Classic Laser Biomedical Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205-7199, USA.
J Biomed Opt. 2006 Sep-Oct;11(5):054034. doi: 10.1117/1.2355666.
In vivo, label-free, high-speed (up to 10,000 with the potential for 40,000 frames per second), high-resolution (up to 300 nm) real-time continuous imaging with successive framing of circulating individual erythrocytes, leukocytes, and platelets in fast blood flow is developed. This technique, used in an animal model, reveals the extremely high dynamic deformability of erythrocytes in natural flow. Potential applications of this technique are discussed with focus on time-resolved monitoring of the cell deformation dynamics in the native biological environment, which may have diagnostic value for the early diagnosis of diseases.
在体内实现了无标记、高速（每秒可达10,000帧，潜在可达40,000帧）、高分辨率（高达300纳米）的实时连续成像，能够连续逐帧记录快速血流中单个循环红细胞、白细胞和血小板。在动物模型中使用该技术，揭示了红细胞在自然流动中的极高动态变形能力。本文讨论了该技术的潜在应用，重点是在天然生物环境中对细胞变形动力学进行时间分辨监测，这可能对疾病的早期诊断具有诊断价值。
