Chaigneau Emmanuelle, Roche Morgane, Charpak Serge
INSERM U1128, Laboratory of Neurophysiology and New Microscopy, Université Paris Descartes, Paris, France.
Front Neurosci. 2019 Jun 28;13:644. doi: 10.3389/fnins.2019.00644. eCollection 2019.
Two-photon laser scanning microscopy is widely used to measure blood hemodynamics in brain blood vessels. Still, the algorithms used so far to extract red blood cell (RBC) size and velocity from line-scan acquisitions have ignored the extent to which scanning speed influences the measurements. Here, we used a theoretical approach that takes into account the velocity and direction of both scanning mirrors and RBCs during acquisition to provide an algorithm that measures the real RBC size and velocity. We validate our approach in brain vessels of anesthetized mice, and demonstrate that it corrects online measurement errors that can reach several 10s of percent as well as data previously acquired. To conclude, our analysis allows unbiased comparisons of blood hemodynamic parameters from brain capillaries and large vessels in control and pathological animal models.
双光子激光扫描显微镜被广泛用于测量脑血管中的血液动力学。然而，迄今为止用于从线扫描采集中提取红细胞（RBC）大小和速度的算法忽略了扫描速度对测量的影响程度。在此，我们采用一种理论方法，该方法在采集过程中考虑了扫描镜和红细胞的速度与方向，以提供一种测量真实红细胞大小和速度的算法。我们在麻醉小鼠的脑血管中验证了我们的方法，并证明它纠正了高达百分之几十的在线测量误差以及先前采集的数据。总之，我们的分析允许在对照和病理动物模型中对脑毛细血管和大血管的血液动力学参数进行无偏比较。
