Wang Yongchao, Xi Lei
School of Physics, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, China.
Vis Comput Ind Biomed Art. 2021 May 26;4(1):15. doi: 10.1186/s42492-021-00081-1.
Photoacoustic (PA) microscopy is being increasingly used to visualize the microcirculation of the brain cortex at the micron level in living rodents. By combining it with long-term cranial window techniques, vasculature can be monitored over a period of days extending to months through a field of view. To fulfill the requirements of long-term in vivo PA imaging, the cranial window must involve a simple and rapid surgical procedure, biological compatibility, and sufficient optical-acoustic transparency, which are major challenges. Recently, several cranial window techniques have been reported for longitudinal PA imaging. Here, the development of chronic cranial windows for PA imaging is reviewed and its technical details are discussed, including window installation, imaging quality, and longitudinal stability.
光声（PA）显微镜越来越多地用于在活体啮齿动物中以微米级可视化大脑皮层的微循环。通过将其与长期颅骨开窗技术相结合，可以在长达数月的时间内通过一个视野监测血管系统。为了满足长期体内PA成像的要求，颅骨开窗必须涉及简单快速的外科手术、生物相容性和足够的光声透明度，而这是主要挑战。最近，已经报道了几种用于纵向PA成像的颅骨开窗技术。在此，对用于PA成像的慢性颅骨开窗的发展进行综述，并讨论其技术细节，包括窗口安装、成像质量和纵向稳定性。
