Bhaskar N, Sudeepa E S, Rashmi H N, Tamil Selvi A
Department of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, Mysore 570 020, India.
Bioresour Technol. 2007 Oct;98(14):2758-64. doi: 10.1016/j.biortech.2006.09.033. Epub 2006 Nov 7.
Bacillus proteolyticus CFR3001 isolated from fish processing wastes (both fresh water and marine) produced an alkaline protease. The optimum conditions for cell growth and protease production were 37 degrees C, 96 h, agitation speed of 100 rpm and medium pH 9. The partially purified protease obtained from had specific activity of 22.05 at 37 degrees C was active between 40 degrees C and 50 degrees C and lost >20% of its activity around 60 degrees C. Its molecular weight was approximately 29 kDa and it inhibited the growth of several pathogenic organisms such as Escherichia coli, Listeria monocytogenes, Bacillus cereus and Yersinia enterocolytica. The scanning electron microscopy (SEM) studies revealed that the protease produced by B. proteolyticus CFR3001 lysed the cells of these pathogenic bacteria.
从鱼类加工废料（淡水和海水）中分离出的解蛋白芽孢杆菌CFR3001可产生一种碱性蛋白酶。细胞生长和蛋白酶产生的最佳条件为37℃、96小时、搅拌速度100转/分钟和培养基pH值9。从该菌获得的部分纯化蛋白酶在37℃时的比活性为22.05，在40℃至50℃之间具有活性，在60℃左右活性损失超过20%。其分子量约为29 kDa，可抑制几种致病微生物的生长，如大肠杆菌、单核细胞增生李斯特菌、蜡样芽孢杆菌和小肠结肠炎耶尔森菌。扫描电子显微镜（SEM）研究表明，解蛋白芽孢杆菌CFR3001产生的蛋白酶可裂解这些病原菌的细胞。
