School of Science, RMIT University, Bundoora, Melbourne, VIC 3083, Australia.
Molecules. 2021 May 24;26(11):3141. doi: 10.3390/molecules26113141.
Although axenic microbial cultures form the basis of many large successful industrial biotechnologies, the production of single commercial microbial strains for use in large environmental biotechnologies such as wastewater treatment has proved less successful. This study aimed to evaluate the potential of the co-culture of two halophilic bacteria, sp. and for enhanced protease activity. The co-culture was significantly more productive than monoculture (1.6-2.0 times more growth), with being predominant (64%). In terms of protease activity, enhanced total activity (1.8-2.4 times) was observed in the co-culture. Importantly, protease activity in the co-culture was found to remain active over a much broader range of environmental conditions (temperature 25 °C to 60 °C, pH 4-12, and 10-30% salinity, respectively). This study confirms that the co-culturing of halophilic bacteria represents an economical approach as it resulted in both increased biomass and protease production, the latter which showed activity over arange of environmental conditions.
尽管无菌微生物培养是许多大型成功工业生物技术的基础，但生产单一商业微生物菌株用于大型环境生物技术，如废水处理，已被证明不太成功。本研究旨在评估两种嗜盐细菌， sp. 和 共培养以提高蛋白酶活性的潜力。与单培养相比，共培养的产量显著更高（生长速度快 1.6-2.0 倍），其中 占优势（64%）。在蛋白酶活性方面，共培养观察到总活性增强（1.8-2.4 倍）。重要的是，共培养中的蛋白酶活性在更广泛的环境条件下保持活性（温度分别为 25°C 至 60°C、pH 值为 4-12 以及盐度为 10-30%）。本研究证实，嗜盐细菌的共培养是一种经济有效的方法，因为它不仅增加了生物量，还提高了蛋白酶的产量，后者在一系列环境条件下均具有活性。
