Allender Matthew C, Fry Michael M, Irizarry Armando R, Craig Linden, Johnson April J, Jones Michael
University of Illinois, College of Veterinary Medicine, 2001 South Lincoln Ave., Urbana, Illinois 61802, USA.
J Wildl Dis. 2006 Jul;42(3):677-84. doi: 10.7589/0090-3558-42.3.677.
A free-ranging adult female eastern box turtle (Terrapene carolina carolina) was presented to the University of Tennessee in October 2003 because of suspected trauma and blindness. Physical examination revealed lethargy, clear ocular and nasal discharges, and white oral and laryngeal plaques. Intracytoplasmic inclusions within heterophils and large mononuclear leukocytes were observed on routine blood smear examination. Postmortem findings included necrosis of epithelial and parenchymal cells with intracytoplasmic inclusions. Ultrastructurally, the leukocyte inclusions consisted of variably electron-dense granular material and viral particles consistent with the Iridoviridae family of viruses. The virus shared 100% sequence identity to a 420-base pair sequence of frog virus 3 (family Iridoviridae, genus Ranavirus) as determined by polymerase chain reaction and gene sequencing targeting a portion of the Ranavirus major capsid protein gene.
2003年10月，一只成年野生东部箱龟（卡罗莱纳箱龟指名亚种，Terrapene carolina carolina）因疑似外伤和失明被送至田纳西大学。体格检查发现其嗜睡、眼鼻有清亮分泌物、口腔和喉部有白色斑块。常规血液涂片检查发现嗜中性粒细胞和大型单核白细胞内有胞浆内包涵体。尸检结果包括上皮细胞和实质细胞坏死以及胞浆内包涵体。超微结构检查显示，白细胞包涵体由电子密度可变的颗粒物质和与虹彩病毒科病毒一致的病毒颗粒组成。通过聚合酶链反应和针对蛙病毒3（虹彩病毒科，蛙病毒属）主要衣壳蛋白基因一部分的基因测序确定，该病毒与蛙病毒3的一段420碱基对序列具有100%的序列同一性。
