Jendza J A, Dilger R N, Sands J S, Adeola O
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907-2054, USA.
J Anim Sci. 2006 Dec;84(12):3364-74. doi: 10.2527/jas.2006-212.
Two studies were conducted to determine the efficacy of an Escherichia coli-derived phytase (ECP) and its equivalency relative to inorganic phosphorus (iP) from monosodium phosphate (MSP). In Exp. 1, one thousand two hundred 1-d-old male broilers were used in a 42-d trial to assess the effect of ECP and iP supplementation on growth performance and nutrient digestibility. Dietary treatments were based on corn-soybean meal basal diets (BD) containing 239 and 221 g of CP, 8.2 and 6.6 g of Ca, and 2.4 and 1.5 g of nonphytate P (nPP) per kg for the starter and grower phases, respectively. Treatments consisted of the BD; the BD + 0.6, 1.2, or 1.8 g of iP from MSP per kg; and the BD + 250, 500, 750, or 1,000 phytase units (FTU) of ECP per kg. Increasing levels of MSP improved gain, gain:feed, and tibia ash (linear, P < 0.01). Increasing levels of ECP improved gain, gain:feed, tibia ash (linear, P < 0.01), apparent ileal digestibility of P, N, Arg, His, Phe, and Trp at d 21 (linear, P < 0.05), and apparent retention of P at d 21 (linear, P < 0.05). Increasing levels of ECP decreased apparent retention of energy (linear, P < 0.01). Five hundred FTU of ECP per kg was determined to be equivalent to the addition of 0.72, 0.78, and 1.19 g of iP from MSP per kg in broiler diets based on gain, feed intake, and bone ash, respectively. In Exp. 2, forty-eight 10-kg pigs were used in a 28-d trial to assess the effect of ECP and iP supplementation on growth performance and nutrient digestibility. Dietary treatments consisted of a positive control containing 6.1 and 3.5 g of Ca and nPP, respectively, per kg; a negative control (NC) containing 4.8 and 1.7 g of Ca and nPP, respectively, per kg; the NC diet plus 0.4, 0.8, or 1.2 g of iP from MSP per kg; and the NC diet plus 500, 750, or 1,000 FTU of ECP per kg. Daily gain improved (linear, P < 0.05) with ECP addition, as did apparent digestibility of Ca and P (linear, P < 0.01). Five hundred FTU of ECP per kg was determined to be equivalent to the addition of 0.49 and 1.00 g of iP from MSP per kg in starter pigs diets, based on ADG and bone ash, respectively.
进行了两项研究，以确定源自大肠杆菌的植酸酶（ECP）的功效及其相对于磷酸二氢钠（MSP）中无机磷（iP）的等效性。在试验1中，1200只1日龄雄性肉鸡用于为期42天的试验，以评估添加ECP和iP对生长性能和养分消化率的影响。日粮处理基于玉米-豆粕基础日粮（BD），育雏期和生长期每千克分别含有239和221克粗蛋白、8.2和6.6克钙以及2.4和1.5克非植酸磷（nPP）。处理包括基础日粮；基础日粮+每千克0.6、1.2或1.8克来自MSP的iP；以及基础日粮+每千克250、500、750或1000植酸酶单位（FTU）的ECP。MSP添加水平的提高改善了增重、料重比和胫骨灰分（呈线性，P<0.01）。ECP添加水平的提高改善了增重、料重比、胫骨灰分（呈线性，P<0.01）以及21日龄时磷、氮、精氨酸、组氨酸、苯丙氨酸和色氨酸的回肠表观消化率（呈线性，P<0.05），还有21日龄时磷的表观存留率（呈线性，P<0.05）。ECP添加水平的提高降低了能量的表观存留率（呈线性，P<0.01）。基于增重、采食量和骨灰分，确定每千克日粮添加500 FTU的ECP分别相当于添加每千克0.72、0.78和1.19克来自MSP的iP。在试验2中，48头10千克的猪用于为期28天的试验，以评估添加ECP和iP对生长性能和养分消化率的影响。日粮处理包括每千克分别含有6.1和3.5克钙和nPP的正对照；每千克分别含有4.8和1.7克钙和nPP的负对照（NC）；NC日粮+每千克0.4、0.8或1.2克来自MSP的iP；以及NC日粮+每千克500、750或1000 FTU的ECP。添加ECP后日增重提高（呈线性，P<0.05），钙和磷的表观消化率也提高（呈线性，P<0.01）。基于平均日增重和骨灰分，确定每千克日粮添加500 FTU的ECP分别相当于在仔猪日粮中添加每千克0.49和1.00克来自MSP的iP。
