从木质部和韧皮部汁液中提取蛋白质。
Protein extraction from xylem and phloem sap.
作者信息
Kehr Julia, Rep Martijn
机构信息
Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany.
出版信息
Methods Mol Biol. 2007;355:27-35. doi: 10.1385/1-59745-227-0:27.
PMID:17093299
Abstract
It is well known that phloem and xylem vessels transport small nutrient molecules over long distances in higher plants. The finding that proteins also occur in both transport fluids was unexpected, and the function of most of these proteins is not yet well understood. This chapter outlines how proteins can be obtained and purified from xylem and phloem saps to perform subsequent proteomic analyses.
摘要
众所周知，在高等植物中，韧皮部和木质部导管能将小营养分子远距离运输。蛋白质也存在于这两种运输液中的这一发现出乎意料，而且这些蛋白质中大多数的功能尚未被充分了解。本章概述了如何从木质部和韧皮部汁液中获取和纯化蛋白质，以便进行后续的蛋白质组学分析。
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