Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.
J Sep Sci. 2020 Jan;43(1):271-284. doi: 10.1002/jssc.201900844. Epub 2019 Dec 4.
Plant vascular tissue is essential for the exchange of water, nutrients, metabolic products, and signals among distant organs in cormophytes. The compositions of phloem and xylem saps are highly dependent on many internal and external factors, and thus their analysis provides a valuable insight into plant physiology, growth, and development as well as nutrition status or presence of biotic or abiotic stresses. Capillary electrophoresis characterized by highly efficient separations and minuscule sample requirements represents a suitable analytical technique for this purpose because the sap constitutes a complex mixture with generally minimal availability. This review aims at providing a comprehensive overview of published capillary electrophoretic methods for the analysis of primary components present in the phloem and xylem saps of higher plants.
植物维管束组织对于维管植物中远距离器官之间的水、养分、代谢产物和信号的交换至关重要。韧皮部和木质部汁液的成分高度依赖于许多内部和外部因素,因此对其进行分析可以深入了解植物的生理、生长和发育,以及营养状况或是否存在生物或非生物胁迫。毛细管电泳具有高效分离和微量样品需求的特点,是一种合适的分析技术,因为汁液是一种复杂的混合物,通常可用性很低。本文旨在全面概述已发表的毛细管电泳方法,用于分析高等植物韧皮部和木质部汁液中存在的主要成分。