Yoshikawa Takaki, Tsuburaya Akira, Miyagi Yohei, Sekiguchi Hironobu, Kimura Masayuki, Cho Haruhiko, Kobayashi Osamu
Department of Gastrointestinal Surgery, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-Ku, Yokohama 241-0815, Japan.
Anticancer Res. 2006 Sep-Oct;26(5B):3849-53.
Gastric cancer often metastasizes to the peritoneal cavity in which tumor cells are exposed to hypoxia without systemic circulation. Hypoxia-inducible factor-1 (HIF-1) and its target gene, vascular endothelial growth factor (VEGF), may play a role in the development of peritoneal metastases.
The mRNA levels of HIF-1 alpha and VEGF were examined in 21 normal gastric mucosa, 158 primary tumors and 18 peritoneal metastases by quantitative RT-PCR.
HIF-1 alpha and VEGF were significantly up-regulated in the peritoneal metastases compared with those of the normal mucosa and the primary tumors. A positive correlation between HIF-1 alpha and VEGF was observed in the peritoneal dissemination.
These results suggest that HIF-1 alpha and its target gene, VEGF, were up-regulated in the intraperitoneal tumors but not in the primary cancers of the stomach. Different microenvironments may influence the expressions of these genes.
胃癌常转移至腹腔，肿瘤细胞在腹腔中暴露于缺氧环境且无全身循环。缺氧诱导因子-1（HIF-1）及其靶基因血管内皮生长因子（VEGF）可能在腹膜转移的发生中起作用。
采用定量逆转录聚合酶链反应检测21例正常胃黏膜、158例原发性肿瘤和18例腹膜转移灶中HIF-1α和VEGF的mRNA水平。
与正常黏膜和原发性肿瘤相比，腹膜转移灶中HIF-1α和VEGF显著上调。在腹膜播散中观察到HIF-1α与VEGF呈正相关。
这些结果表明，HIF-1α及其靶基因VEGF在腹腔内肿瘤中上调，但在胃癌原发性肿瘤中未上调。不同的微环境可能影响这些基因的表达。
