Berger Jennifer, Mueller-Holzner Elisabeth, Fiegl Heidi, Marth Christian, Daxenbichler Guenter
Department of Obstetrics and Gynecology, Innsbruck University Hospital, Innsbruck, Austria.
Anticancer Res. 2006 Sep-Oct;26(5B):3855-60.
The expressions of three mRNA markers were correlated with the results of extensive histopathological examination of a total of 290 axillary lymph nodes from 29 breast carcinoma patients. Included were two established markers for breast cancer (cytokeratin-19 and mammaglobin) and the novel marker DNA methyltransferase 3b (DNMT3b). DNMT3b was significantly overexpressed in breast cancer compared to normal breast tissue. The expression of the three markers in axillary lymph nodes was determined using quantitative real-time RT-PCR. DNMT3b expression showed a specificity of more than 99%, which was comparable to that of cytokeratin-19 and better than that of mammaglobin. The sensitivity of RT-PCR relative to histopathology was highest for cytokeratin-19 (96%), followed by DNMT3b (88%) and mammaglobin (68%). The overall agreement of histological and RT-PCR results was 96-99%. The results indicate that expression analysis of marker genes by quantitative RT-PCR can be a useful tool for lymph node diagnosis in breast cancer.
对29例乳腺癌患者共290个腋窝淋巴结进行广泛组织病理学检查，结果显示三种mRNA标志物的表达与之相关。其中包括两种已确立的乳腺癌标志物（细胞角蛋白-19和乳腺珠蛋白）以及新型标志物DNA甲基转移酶3b（DNMT3b）。与正常乳腺组织相比，DNMT3b在乳腺癌中显著过表达。采用定量实时逆转录聚合酶链反应（RT-PCR）测定腋窝淋巴结中这三种标志物的表达。DNMT3b表达的特异性超过99%，与细胞角蛋白-19相当，优于乳腺珠蛋白。RT-PCR相对于组织病理学的敏感性，细胞角蛋白-19最高（96%），其次是DNMT3b（88%）和乳腺珠蛋白（68%）。组织学和RT-PCR结果的总体一致性为96%-99%。结果表明，通过定量RT-PCR分析标志物基因的表达可作为乳腺癌淋巴结诊断的有用工具。
