Faculty of Natural Science and Mathematics, Universidad del Rosario, Bogotá DC, Colombia.
School of Medicine and Health Sciences, Universidad del Rosario, Bogotá DC, Colombia.
PLoS One. 2019 May 23;14(5):e0216989. doi: 10.1371/journal.pone.0216989. eCollection 2019.
This meta-analysis presents evidence regarding the diagnostic accuracy of mammaglobin detected using the RT-PCR technique, related to the presence of sentinel node metastasis in breast cancer patients.
The following databases were consulted: Cochrane, Lilacs, Scielo, Hinary, PubMed, Elsevier, Embase, ProQuest, the Universidad del Rosario´s Centro de Recursos Para el Aprendizaje y la Investigación (CRAI-UR) [Resource Center for Learning and Research], and the Google Scholar search engine. The quality of the studies was assessed using the QUADAS-2 and CASpe tools. The selected studies presented the necessary data to calculate diagnostic validity index of mammaglobin detection using RT-PCR, compared with the reference standard test. Global values for the sensitivity, specificity, positive predictive value, negative predictive value, probability ratios, diagnostic ORs, and summary ROC curves of this meta-analysis were obtained using the Meta-DiSc 1.4 program.
Initially, 731 articles were obtained; but only 25 were included in the meta-analysis. Sensitivity was 84% (95% CI: 83% - 86%), and specificity was 92% (95% CI: 91% - 93%). Positive and negative predictive values were 9.26 (95% CI: 6.47-13.26) and 0.17 (95% CI: 0.13-0.23), respectively. The diagnostic OR was 66.34 (95% CI: 42.52-103.52). The predictive area under the sROC curve was 94.78 (Q = 0.8876).
The evaluated diagnostic index showed that the expression of the mammaglobin biomarker has diagnostic prediction for detecting lymph node metastasis in breast cancer patients, when analyzed using RT-PCR, although more than 50% heterogeneity was found.
本荟萃分析提供了使用 RT-PCR 技术检测乳球蛋白在乳腺癌患者前哨淋巴结转移中的诊断准确性的证据。
检索了 Cochrane、Lilacs、Scielo、Hinary、PubMed、Elsevier、Embase、ProQuest、罗萨里奥大学学习与研究资源中心（CRAI-UR）和谷歌学术搜索引擎。使用 QUADAS-2 和 CASpe 工具评估研究质量。选择的研究提供了使用 RT-PCR 检测乳球蛋白的诊断有效性指数的必要数据，并与参考标准测试进行了比较。使用 Meta-DiSc 1.4 程序获得了该荟萃分析的敏感性、特异性、阳性预测值、阴性预测值、概率比、诊断优势比和汇总 ROC 曲线的总体值。
最初获得了 731 篇文章，但只有 25 篇被纳入荟萃分析。敏感性为 84%（95%CI：83%-86%），特异性为 92%（95%CI：91%-93%）。阳性和阴性预测值分别为 9.26（95%CI：6.47-13.26）和 0.17（95%CI：0.13-0.23）。诊断优势比为 66.34（95%CI：42.52-103.52）。sROC 曲线下的预测面积为 94.78（Q=0.8876）。
评估的诊断指数表明，当使用 RT-PCR 分析时，乳球蛋白生物标志物的表达对检测乳腺癌患者的淋巴结转移具有诊断预测价值，尽管存在超过 50%的异质性。
