Liakakos Theodore, Troupis Theodore, Ghiconti Ioanna, Triantafyllidis Spyros, Macheras Anastasios, Karatzas Gabriel, Pavlakis Kitty
Third Department of Surgery, University of Athens, ATTIKON, University General Hospital, 1 Rimini St., Haidari, 12462 Athens, Greece.
Anticancer Res. 2006 Sep-Oct;26(5B):3899-903.
The aim of this study was to investigate the expression of thymidine phosphorylase (TP), a known angiogenic factor for endothelial cells, in gastric carcinoma cells and tumor-associated stromal cells.
Sixty-six gastric carcinomas were studied. TP expression was assessed with the P-GF.44C mouse monoclonal antibody using the avidin-biotin immunoperoxidase technique. The results were correlated with several clinicopathological parameters and patient survival.
TP expression in cancer cells was related to the age of the patients and the overall survival. When TP was expressed in tumor-associated stromal cells, it was statistically related to poorly-differentiated tumors. Statistical analysis revealed no relationship between TP expression and any of the clinicopathological parameters under evaluation, when considering stromal TP immunoreactivity separately for stromal fibroblasts and associated inflammatory cells, or when considering the tumors as TP-positive, irrespective of the tissue localization of the enzyme.
TP seems to be a prognostic indicator in gastric cancer patients only when the enzyme is located in tumor cells. The different impacts of TP expression in tumor cells and associated stromal cells might indicate that the enzyme may have more than one function involved in tumor growth.
本研究旨在调查胸苷磷酸化酶（TP），一种已知的内皮细胞血管生成因子，在胃癌细胞和肿瘤相关基质细胞中的表达情况。
对66例胃癌进行了研究。使用抗生物素蛋白-生物素免疫过氧化物酶技术，用P-GF.44C小鼠单克隆抗体评估TP表达。结果与几个临床病理参数和患者生存率相关。
癌细胞中的TP表达与患者年龄和总生存率有关。当TP在肿瘤相关基质细胞中表达时，与低分化肿瘤在统计学上相关。统计分析显示，当分别考虑基质成纤维细胞和相关炎症细胞的基质TP免疫反应性时，或者当将肿瘤视为TP阳性时，无论该酶在组织中的定位如何，TP表达与所评估的任何临床病理参数之间均无关联。
仅当该酶位于肿瘤细胞中时，TP似乎才是胃癌患者的预后指标。TP在肿瘤细胞和相关基质细胞中的不同影响可能表明该酶在肿瘤生长中可能具有多种功能。
