Hakozaki Michiyuki, Hojo Hiroshi, Sato Michiko, Tajino Takahiro, Yamada Hitoshi, Kikuchi Shinichi, Abe Masafumi
First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima-shi, Fukushima 960-1295, Japan.
Anticancer Res. 2006 Sep-Oct;26(5A):3393-401.
Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarcomas in adults. In order to improve its aggressive course or prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of UPS are required.
A new human UPS cell line (FPS-1) was established from UPS of the upper arm of a 79-year-old man. The cell line has been maintained for over 14 months with more than 60 passages. FPS-1 cells were characterized using molecular biological methods.
FPS-1 cells showed the same morphological and immunophenotypical characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a nonsense mutation in exon 6 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in FPS-1 cells.
FPS-1 cells might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for UPS with EGFR or COX-2 expression.
未分化多形性肉瘤（UPS）是成人中最常见的软组织肉瘤之一。为改善其侵袭性病程或预后并建立新的治疗方法，需要对UPS进行分子遗传学和生物学特征分析。
从一名79岁男性上臂的UPS中建立了一种新的人UPS细胞系（FPS-1）。该细胞系已传代60多次，维持培养超过14个月。使用分子生物学方法对FPS-1细胞进行特征分析。
FPS-1细胞表现出与原发肿瘤相同的形态学和免疫表型特征。细胞遗传学和分子分析显示p53基因第6外显子存在无义突变。表皮生长因子受体（EGFR）和环氧合酶-2（COX-2）在FPS-1细胞中表达。
FPS-1细胞可能有助于研究具有EGFR或COX-2表达的UPS的生物学行为，并开发新的分子靶向抗肿瘤药物。
