Li Guan-Cheng, Wang Zhao-Yi
Zhuhai Campus, Zunyi Medical College, Zhuhai, Guangdong 519041, PR China.
Anticancer Res. 2006 Sep-Oct;26(5A):3555-60.
Epithelial-mesenchymal transition (EMT) is a component of several morphogenetic and organogenetic processes during embryonic development. EMT is a strictly regulated process and its aberrant regulation may contribute to cancer progression and metastasis. The retinoblastoma suppressor (Rb)-associated protein 46 (RbAp46) is a component of the histone-modifying and -remodeling complexes.
Western blot and immunofluorescent assays with epithelial and mesenchymal markers and cell migration and invasion assays were employed.
Mammary epithelial cells, MCF10AT3B, that express recombinant RbAp46 exhibited an EMT associated with the down-regulation of epithelial markers and up-regulation of mesenchymal markers, as well as an increased migration and invasion phenotype.
Rbap46 may be an important player in EMT during embryonic development. Dysregulated RbAp46 expression may contribute to cancer progression and metastasis.
上皮-间质转化（EMT）是胚胎发育过程中几种形态发生和器官发生过程的一个组成部分。EMT是一个严格调控的过程，其异常调控可能导致癌症进展和转移。视网膜母细胞瘤抑制蛋白（Rb）相关蛋白46（RbAp46）是组蛋白修饰和重塑复合物的一个组成部分。
采用上皮和间质标志物的蛋白质印迹法和免疫荧光法以及细胞迁移和侵袭试验。
表达重组RbAp46的乳腺上皮细胞MCF10AT3B表现出与上皮标志物下调、间质标志物上调相关的EMT，以及迁移和侵袭表型增加。
Rbap46可能是胚胎发育过程中EMT的一个重要参与者。RbAp46表达失调可能导致癌症进展和转移。
