Dormán György, Puskás László G, Fehér Liliána Z, Hackler László, Lorincz Zsolt, Lang Csaba, Urge László, Darvas Ferenc
ComGenex, Budapest, Záhony.
Acta Pharm Hung. 2006;76(1):3-9.
The simultaneous identification of disease-specific protein targets and their small molecule binding partners, suitable as drug candidates, could radically reduce the timeline and costs of drug discovery and development. Comparative chemical proteomics provides a novel approach to achieve this goal through rapid detection of overexpressed proteins in diseased samples by the application of small molecule microarrays. The interacting small molecules enables direct affinity-based isolation and identification of the proteins. In the present paper we report comparative chemical proteomics studies on melanocytes and melanoma cell-lines, which led to the identification of 3 overexpressed proteins (e.g. -tubulin) together with their small molecule binding partner.
同时鉴定疾病特异性蛋白质靶点及其小分子结合伴侣（适合作为候选药物），可能会从根本上缩短药物发现和开发的时间线并降低成本。比较化学蛋白质组学提供了一种新方法，通过应用小分子微阵列快速检测患病样本中过表达的蛋白质来实现这一目标。相互作用的小分子能够基于直接亲和力分离和鉴定蛋白质。在本文中，我们报告了对黑素细胞和黑色素瘤细胞系的比较化学蛋白质组学研究，该研究鉴定出了3种过表达蛋白质（如β-微管蛋白）及其小分子结合伴侣。
