Ge Hui, Wang Yi-Min, Cao Yan-Yan, Zhang Xiu-Feng, Li Yan, Guo Wei, Wang Na, Zhang Jian-Hui
Laboratory of Molecular Biology, Hebei Provincial Cancer Institute, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050011, P. R. China.
Ai Zheng. 2006 Nov;25(11):1351-5.
BACKGROUND & OBJECTIVE: p73 is a tumor suppressor gene. The two polymorphisms in the non-coding region of the exon 2 of p73 gene, named G4C14-A4T14, can form a stem-loop structure, and may influence p73 expression. This study was to investigate the correlation of p73 G4C14-A4T14 polymorphisms to susceptibilities to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in a high incidence region of Hebei Province.
We conducted a population-based case-control study in 348 ESCC patients, 259 GCA patients, and 630 healthy controls. p73 genotypes were determined by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP).
Family history of upper gastrointestinal cancer (UGIC) significantly increased the risk of developing ESCC and GCA [the age, sex, and smoking status adjusted odds ratio (OR)=1.68, 95% confidence interval (CI)=1.28-2.20; OR=1.68, 95% CI=1.24-2.26, respectively]. The overall distribution of p73 genotype and allelotype in cancer patients and controls were not significantly different. Stratification analysis by smoking status and family history of UGIC found that the GC/AT genotype significantly increased the risk of developing GCA among the subjects without family history of UGIC (OR=1.71, 95% CI=1.14-2.57), while p73 G4C14-A4T14 polymorphisms did not increase the risk of developing ESCC and GCA in other subgroups.
In p73 G4C14-A4T14 polymorphisms, the GC/AT genotype increases the risk of developing GCA among the subjects without family history of UGIC.
p73是一种肿瘤抑制基因。p73基因外显子2非编码区的两个多态性位点，即G4C14-A4T14，可形成茎环结构，并可能影响p73的表达。本研究旨在探讨河北省高发地区p73基因G4C14-A4T14多态性与食管鳞状细胞癌（ESCC）和贲门腺癌（GCA）易感性的相关性。
我们在348例ESCC患者、259例GCA患者和630例健康对照中开展了一项基于人群的病例对照研究。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法测定p73基因型。
上消化道癌（UGIC）家族史显著增加了患ESCC和GCA的风险[年龄、性别和吸烟状况校正后的优势比（OR）=1.68，95%置信区间（CI）=1.28-2.20；OR=1.68，95%CI=1.24-2.26]。癌症患者和对照中p73基因型和等位基因型的总体分布无显著差异。按吸烟状况和UGIC家族史进行分层分析发现，GC/AT基因型在无UGIC家族史的受试者中显著增加了患GCA的风险（OR=1.71，95%CI=1.14-2.57），而p73基因G4C14-A4T14多态性在其他亚组中并未增加患ESCC和GCA的风险。
在p73基因G4C14-A4T14多态性中，GC/AT基因型在无UGIC家族史的受试者中增加了患GCA的风险。
