Barritt Jason, Luna Martha, Duke Marlena, Grunfeld Lawrence, Mukherjee Tanmoy, Sandler Benjamin, Copperman Alan B
Department of Obstetrics and Gynecology, and Department of Reproductive Endocrinology and Infertility, Mount Sinai School of Medicine, New York, New York, USA.
Fertil Steril. 2007 Jan;87(1):189.e13-7. doi: 10.1016/j.fertnstert.2006.04.052. Epub 2006 Nov 7.
To determine the clinical potential of donor-oocyte cryopreservation and thaw techniques for recipient patients.
Institutional review board-approved prospective study of donor oocyte cryopreservation.
A large, private infertility center.
PATIENT(S): Four anonymous oocyte donors underwent ovarian hyperstimulation for the purpose of oocyte retrieval and cryopreservation. The oocytes were subsequently thawed, fertilized, and transferred to 4 recipient patients.
INTERVENTION(S): Oocytes were obtained from young donor patients and were cryopreserved with a slow freeze/rapid thaw protocol in which 1,2-propanediol (PrOH) and sucrose were used as cryoprotectants. Oocytes that survived were inseminated using intracytoplasmic sperm injection (ICSI). Resulting embryos were replaced into the recipient patients on the third day post-insemination.
MAIN OUTCOME MEASURE(S): Post-thaw survival rate, fertilization rate, cleavage rate, implantation and clinical pregnancy rates.
RESULT(S): A total of 79 metaphase II oocytes were frozen, stored frozen overnight in liquid nitrogen, and then thawed. The post-thaw survival rate was 86.1%. Normal fertilization following ICSI occurred in 89.7% of the surviving oocytes. Cleavage was observed in 91.8% of normally fertilized oocytes. A total of 23 embryos were transferred to 4 recipient patients. A clinical pregnancy rate of 75% and an implantation rate of 26.1% were achieved.
CONCLUSION(S): Human oocyte cryopreservation is an effective technique that can be applied in clinical situations with high oocyte survival and clinical pregnancy rates expected.
确定供体卵母细胞冷冻保存和解冻技术对受体患者的临床应用潜力。
经机构审查委员会批准的供体卵母细胞冷冻保存前瞻性研究。
一家大型私立不孕不育中心。
4名匿名卵母细胞供体接受了卵巢超刺激，以获取卵母细胞并进行冷冻保存。随后，这些卵母细胞被解冻、受精，并移植给4名受体患者。
从年轻供体患者获取卵母细胞，采用慢速冷冻/快速解冻方案进行冷冻保存，其中使用1,2 - 丙二醇（PrOH）和蔗糖作为冷冻保护剂。存活的卵母细胞采用卵胞浆内单精子注射（ICSI）进行授精。授精后第三天将所得胚胎移植到受体患者体内。
解冻后存活率、受精率、卵裂率、着床率和临床妊娠率。
共冷冻了79个中期II卵母细胞，在液氮中过夜保存后解冻。解冻后存活率为86.1%。ICSI后89.7%的存活卵母细胞实现正常受精。91.8%的正常受精卵母细胞观察到卵裂。共将23个胚胎移植给4名受体患者。临床妊娠率为75%，着床率为26.1%。
人类卵母细胞冷冻保存是一种有效的技术，可应用于预期卵母细胞存活率和临床妊娠率较高的临床情况。
