Ar'Rajab A, Ahrén B, Bengmark S
Department of Surgery, Lund University, Sweden.
Transplantation. 1991 May;51(5):965-7.
Intracellular calcium is an important determinant for cell death in organ hypothermic preservation for transplantation. In this study, we show that prevention of calcium entry improves the result of liver cold storage in UW solution. The isolated perfused rabbit liver was used. After 48 hr of cold storage in UW solution, bile production was reduced by 70% (P less than 0.005). However, by adding the calcium channel blockers verapamil or nifedipine (40 microM) to the UW solution, this reduction was abolished, and the livers produced the same amount of bile as unpreserved livers. Furthermore, addition of the calcium channel activator, BAY K8644 (40 microM), to the UW solution, reduced bile production by 50% (P less than 0.01) already after preservation for 24 hr. We conclude that calcium entry is of importance for liver function after preservation and cold storage, and that including a calcium channel blocker to the preservation solution makes long-term liver preservation safer.
细胞内钙是器官低温保存用于移植时细胞死亡的一个重要决定因素。在本研究中，我们表明阻止钙内流可改善肝脏在UW液中的冷藏效果。采用离体灌注兔肝。在UW液中冷藏48小时后，胆汁分泌减少了70%（P<0.005）。然而，通过向UW液中添加钙通道阻滞剂维拉帕米或硝苯地平（40微摩尔），这种减少被消除，肝脏分泌的胆汁量与未保存的肝脏相同。此外，在UW液中添加钙通道激活剂BAY K8644（40微摩尔），在保存24小时后胆汁分泌就减少了50%（P<0.01）。我们得出结论，钙内流对保存和冷藏后的肝功能很重要，并且在保存液中加入钙通道阻滞剂可使肝脏长期保存更安全。
