Yoshida Sei, Handa Yutaka, Suzuki Toshihiko, Ogawa Michinaga, Suzuki Masato, Tamai Asuka, Abe Akio, Katayama Eisaku, Sasakawa Chihiro
Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Science. 2006 Nov 10;314(5801):985-9. doi: 10.1126/science.1133174.
Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its alpha-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells.
一些致病细菌实际上会侵入其靶宿主细胞的细胞质。侵入性细菌通过招募肌动蛋白并诱导其聚合来获得移动的推进力。在这里，我们表明，志贺氏菌在细胞质内的移动受到微管的严重阻碍，并且细菌通过III型分泌系统分泌VirA来破坏周围的微管。VirA对微管的降解取决于其α-微管蛋白特异性半胱氨酸蛋白酶样活性。virA突变体在宿主细胞质内不移动，也无法移动到相邻细胞中。
