核酸完整的、序列依赖的折叠态势的直接测量。
Direct measurement of the full, sequence-dependent folding landscape of a nucleic acid.
作者信息
Woodside Michael T, Anthony Peter C, Behnke-Parks William M, Larizadeh Kevan, Herschlag Daniel, Block Steven M
机构信息
National Institute for Nanotechnology, National Research Council of Canada, Edmonton AB, Canada, T6G 2M9.
出版信息
Science. 2006 Nov 10;314(5801):1001-4. doi: 10.1126/science.1133601.
Abstract
Nucleic acid hairpins provide a powerful model system for understanding macromolecular folding, with free-energy landscapes that can be readily manipulated by changing the hairpin sequence. The full shapes of energy landscapes for the reversible folding of DNA hairpins under controlled loads exerted by an optical force clamp were obtained by deconvolution from high-resolution, single-molecule trajectories. The locations and heights of the energy barriers for hairpin folding could be tuned by adjusting the number and location of G:C base pairs, and the presence and position of folding intermediates were controlled by introducing single-nucleotide mismatches.
摘要
核酸发夹为理解大分子折叠提供了一个强大的模型系统，其自由能景观可以通过改变发夹序列轻松操控。通过对高分辨率单分子轨迹进行去卷积，获得了在光镊施加的可控负载下DNA发夹可逆折叠的能量景观的完整形状。通过调整G:C碱基对的数量和位置，可以调节发夹折叠的能垒位置和高度，通过引入单核苷酸错配来控制折叠中间体的存在和位置。
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