Düzgün Nurşen, Duman Türker, Haydardedeoğlu Filiz Ekşi, Tutkak Hüseyin
Department of Clinical Immunology and Rheumatology, Ankara University School of Medicine, Ankara, Turkey.
Clin Rheumatol. 2007 Jun;26(6):915-9. doi: 10.1007/s10067-006-0432-x. Epub 2006 Nov 10.
Acute rheumatic fever (ARF) is a non-suppurative inflammatory disease after group A, beta haemolytic streptococcal pharyngitis. Certain individuals can develop ARF. This finding implies variability in host predisposition to ARF. A variety of studies have linked specific genetic markers with ARF or rheumatic heart disease (RHD) as a sequelae of ARF. For this purpose, we aimed to search the role of polymorphisms in Toll-like receptor-2 and -4 (TLR2 and TLR4) gene in Turkish patients with RHD. This study included a total 84 patients with RHD, ages ranging between 18 and 65, 25 male and 59 female, fulfilling the revised classification criteria of Jones. One hundred forty healthy unrelated persons were selected as a control group. Genotype analysis: DNA was extracted from whole blood. TLR4 gene (Asp 299Gly and Thr399Ile) and TLR2 gene (Arg753Gln and Arg677Trp) polymorphisms were genotyped by the previously reported method.
binary logistic regression models were used. Results were expressed as odds ratios (OR) with corresponding 95% confidence intervals (95% CI). Significant level was predefined at 0.05. There was a significant difference for carrying Ile allele in the 399 position in the patients compared to healthy controls (OR = 5.26, 95% CI, 1.40-19.73, p = 0.014). In the TLR4 gene, Asp 299Gly polymorphism did not reach to a statistically significant value (OR = 3.02). We found no Arg753Gln polymorphism of the TLR2 gene in the patient group. There were three heterozygote samples in the healthy group. We did not detect Arg677Trp polymorphism of the TLR2 gene in both patient and control groups.
急性风湿热（ARF）是A组β溶血性链球菌咽炎后的一种非化脓性炎症性疾病。某些个体可发生ARF。这一发现意味着宿主对ARF的易感性存在差异。各种研究已将特定的基因标记与ARF或作为ARF后遗症的风湿性心脏病（RHD）联系起来。为此，我们旨在探究Toll样受体2和4（TLR2和TLR4）基因多态性在土耳其RHD患者中的作用。本研究共纳入84例RHD患者，年龄在18至65岁之间，男性25例，女性59例，符合琼斯修订分类标准。选择140名健康无关个体作为对照组。基因分型分析：从全血中提取DNA。采用先前报道的方法对TLR4基因（Asp 299Gly和Thr399Ile）和TLR2基因（Arg753Gln和Arg677Trp）多态性进行基因分型。
使用二元逻辑回归模型。结果以比值比（OR）及相应的95%置信区间（95%CI）表示。显著性水平预先设定为0.05。与健康对照组相比，患者中399位携带Ile等位基因存在显著差异（OR = 5.26，95%CI，1.40 - 19.73，p = 0.014）。在TLR4基因中，Asp 299Gly多态性未达到统计学显著值（OR = 3.02）。我们在患者组中未发现TLR2基因的Arg753Gln多态性。健康组中有三个杂合子样本。我们在患者组和对照组中均未检测到TLR2基因的Arg677Trp多态性。
