抗人胎盘谷胱甘肽转移酶（π类）的单克隆抗体。

Monoclonal antibodies against human placental glutathione transferase (class pi).

作者信息

Massoud R, Lo Bello M, De Stefano E, Molino A, Zelaschi D, Federici G

机构信息

Department of Biology, 2nd University of Rome Tor Vergata, Italy.

出版信息

Hybridoma. 1991 Feb;10(1):89-94. doi: 10.1089/hyb.1991.10.89.

DOI:10.1089/hyb.1991.10.89

PMID:1709614

Abstract

Five monoclonal antibodies (MAbs) were produced in a mouse hybridoma system against human placental glutathione transferase (GST pi). Four of these monoclonal antibodies, named 461 to 464, were of immunoglobulin G class, whereas the monoclonal antibody 465 was of IgA class. All these MAbs specifically recognized the glutathione transferase from human placenta (class pi) showing no cross reactivity against the basic and the neutral forms of GST from human liver. When each MAb was incubated with the GST pi, no inhibition of enzymatic activity towards 1-chloro-2,4-dinitrobenzene was observed except for MAb 465 which showed a slight inhibition to a serial dilution of 1:128.

摘要

在小鼠杂交瘤系统中产生了五种针对人胎盘谷胱甘肽转移酶（GST π）的单克隆抗体（MAb）。其中四种单克隆抗体，命名为461至464，属于免疫球蛋白G类，而单克隆抗体465属于IgA类。所有这些单克隆抗体都能特异性识别来自人胎盘的谷胱甘肽转移酶（π类），对人肝脏中碱性和中性形式的GST无交叉反应。当每种单克隆抗体与GST π孵育时，除了单克隆抗体465对1:128系列稀释液表现出轻微抑制外，未观察到对1-氯-2,4-二硝基苯的酶活性有抑制作用。