Vos R M, Lutkeschipholt I J, Boonekamp P M, Schots A, Pomp H, Van Bladeren P J, Koeman J H
Agricultural University, Department of Toxicology, Wageningen, The Netherlands.
Hybridoma. 1989 Aug;8(4):475-80. doi: 10.1089/hyb.1989.8.475.
Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rat isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3. Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.
制备了针对大鼠谷胱甘肽S-转移酶同工酶2-2和3-3的单克隆抗体。在酶联免疫吸附测定（ELISA）和蛋白质印迹法中，三个克隆能够将各自的抗原与其他大鼠同工酶以及人类同工酶特异性区分开来。一个克隆产生了对同工酶2-2特异的抗体，两个杂交瘤对同工酶3-3特异。Balb/c小鼠对谷胱甘肽S-转移酶同工酶1-1和4-4免疫无反应。然而，在一些具有不同H-2单倍型的其他品系小鼠中获得了免疫反应，特别是对于同工酶1-1的CBA/BrARij小鼠和CBA/CaHRij-T6小鼠，以及对于同工酶4-4的CBA/BrARij小鼠，这为获得针对这些谷胱甘肽S-转移酶的额外特异性单克隆抗体提供了前景。
