Goh Kelvin K T, Pinder D Neil, Hall Christopher E, Hemar Yacine
Institute of Food, Nutrition and Human Health and Institute of Fundamental Sciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Biomacromolecules. 2006 Nov;7(11):3098-103. doi: 10.1021/bm060577u.
Polysaccharides isolated from flaxseed meals using ethanol consisted of a soluble ( approximately 7.5% w/w) and an insoluble fraction (2% w/w). The soluble fraction was dialyzed in various salt concentrations and characterized using viscometry and light scattering techniques. Observations using a size-exclusion column coupled to a multiangle laser light scattering (SEC-MALLS) revealed three molecular weight fractions consisting of a small amount ( approximately 17%) of large molecular weight species (1.0 x 10(6)) and a large amount ( approximately 69%) of small molecular weight species (3.1 x 10(5) Da). Dynamic light scattering measurements indicated the presence of very small molecules (hydrodynamic radius approximately 10 nm) and a very large molecular species (hydrodynamic radius in excess of 100 nm); the latter were probably aggregates. The intrinsic viscosity, [eta], of the polysaccharide in Milli-Q water was 1030 +/- 20 mL/g. The viscosity was due largely to the large molecular weight species since viscosity is influenced by the hydrodynamic volume of molecules in solution. The Smidsrod parameter B obtained was approximately 0.018, indicating that the molecules adopted a semi-flexible conformation. This was also indicated by the slope ( approximately 0.56) from the plot of root-mean-square (RMS) radius versus molar mass (M(w)).
使用乙醇从亚麻籽粕中分离得到的多糖由可溶部分（约7.5% w/w）和不溶部分（2% w/w）组成。可溶部分在不同盐浓度下进行透析，并使用粘度测定法和光散射技术进行表征。使用与多角度激光光散射联用的尺寸排阻色谱（SEC-MALLS）观察发现，多糖有三个分子量级分，其中少量（约17%）为大分子物种（1.0×10⁶），大量（约69%）为小分子物种（3.1×10⁵ Da）。动态光散射测量表明存在非常小的分子（流体动力学半径约为10 nm）和非常大的分子物种（流体动力学半径超过100 nm）；后者可能是聚集体。多糖在超纯水（Milli-Q水）中的特性粘度[η]为1030±20 mL/g。粘度主要归因于大分子物种，因为粘度受溶液中分子流体动力学体积的影响。得到的斯米德罗德参数B约为0.018，表明分子呈半柔性构象。这也由均方根（RMS）半径与摩尔质量（M(w)）的关系图的斜率（约0.56）表明。
