Koopman G, Parmentier H K, Schuurman H J, Newman W, Meijer C J, Pals S T
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
J Exp Med. 1991 Jun 1;173(6):1297-304. doi: 10.1084/jem.173.6.1297.
Presentation of antigen in the form of immune complexes to B lymphocytes by follicular dendritic cells (FDC) is considered to be a central step in the generation of memory B cells. During this process, which takes place in the microenvironment of the germinal center, B cells and FDC are in close physical contact. In the present study, we have explored the molecular basis of FDC-B cell interaction by using FDC and B cells derived from human tonsils. We found that FDC express high levels of the adhesion receptors intercellular adhesion molecule 1 (ICAM-1 [CD54]) and vascular cell adhesion molecule 1 (VCAM-1), while the B lymphocytes express lymphocyte function-associated antigen 1 (LFA-1 [CD11a/18]), very late antigen 4 (VLA-4 [CD49d], and CD44. Furthermore, we established that both the LFA-1/ICAM-1 and VLA-4/VCAM-1 adhesion pathways are involved in FDC-B lymphocyte binding, and therefore, these pathways might be essential in affinity selection of B cells and in the formation of B memory cells.
滤泡树突状细胞（FDC）以免疫复合物的形式向B淋巴细胞呈递抗原被认为是记忆B细胞产生过程中的核心步骤。在生发中心微环境中发生的这一过程中，B细胞与FDC存在紧密的物理接触。在本研究中，我们利用源自人扁桃体的FDC和B细胞探索了FDC与B细胞相互作用的分子基础。我们发现FDC高水平表达黏附受体细胞间黏附分子1（ICAM-1 [CD54]）和血管细胞黏附分子1（VCAM-1），而B淋巴细胞表达淋巴细胞功能相关抗原1（LFA-1 [CD11a/18]）、极迟抗原4（VLA-4 [CD49d]）和CD44。此外，我们确定LFA-1/ICAM-1和VLA-4/VCAM-1这两条黏附途径均参与FDC与B淋巴细胞的结合，因此，这些途径可能在B细胞的亲和力选择以及B记忆细胞的形成中至关重要。
