Sun Xian-ce, Piao Feng-yuan, Wang Yi, Xu Yuan-yuan, Li Xin, Li Bing, Jin Ya-ping, Sun Gui-fan
Hygiene Department, Colleges of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116027, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Oct;24(10):605-7.
To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).
The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.
If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).
CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.
评估亚砷酸钠对人角质形成细胞系（HaCaT）中过氧化氢酶（CAT）活性、mRNA及蛋白表达的影响。
采用紫外直接速率法检测过氧化氢酶（CAT）的活性。运用逆转录聚合酶链反应（RT-PCR）检测CAT的mRNA表达，采用蛋白质免疫印迹法检测CAT的蛋白表达。
当细胞用高于5.0微摩尔/升的亚砷酸钠处理时，CAT的活性、mRNA及蛋白表达均显著降低，且呈剂量依赖性（P<0.05）。
亚砷酸钠在转录、翻译及活性水平上抑制CAT。
