Sun Xiance, Li Bing, Li Xin, Wang Yi, Xu Yuanyuan, Jin Yaping, Piao Fengyuan, Sun Guifan
Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, Liaoning, PR China.
Toxicol In Vitro. 2006 Oct;20(7):1139-44. doi: 10.1016/j.tiv.2006.02.008. Epub 2006 Feb 28.
The objective of this research work is to study the effects of sodium arsenite on activity, mRNA and protein expression of catalase (CAT) in established human cell lines of keratinocytes (HaCaT). Based on the AlamarBlue assay, we found that a high level (100 micromol/l) of sodium arsenite inhibited cell viability. The reactive oxygen species (ROS) content in cells was increased in all treated cultures dose-dependently. CAT activity, mRNA expression and protein levels were decreased by 5-20 micromol/l of sodium arsenite. It is highly suggestive that CAT gene expression and protein levels are affected by arsenic.
本研究工作的目的是研究亚砷酸钠对已建立的人角质形成细胞系（HaCaT）中过氧化氢酶（CAT）的活性、mRNA和蛋白质表达的影响。基于alamarBlue检测，我们发现高浓度（100微摩尔/升）的亚砷酸钠会抑制细胞活力。在所有处理的培养物中，细胞内活性氧（ROS）含量呈剂量依赖性增加。5-20微摩尔/升的亚砷酸钠可降低CAT活性、mRNA表达和蛋白质水平。这强烈表明CAT基因表达和蛋白质水平受砷的影响。
