Lestremau François, de Villiers André, Lynen Frédéric, Cooper Andrew, Szucs Roman, Sandra Pat
Pfizer Analytical Research Centre-Ghent University, Krijgslaan 281 S4-bis, B-9000 Ghent, Belgium.
J Chromatogr A. 2007 Jan 5;1138(1-2):120-31. doi: 10.1016/j.chroma.2006.10.042. Epub 2006 Nov 9.
Theoretical aspects of temperature in liquid chromatography (LC) have mostly been studied to elucidate changes in retention behavior of small and large molecules in various solvents. That temperature also plays a significant role in chromatographic performance is less known. Kinetic plots are an established tool to predict chromatographic performance in terms of speed and efficiency that can be obtained with a certain particle size at the maximum attainable column pressure. In this paper, temperature effects on mobile phase viscosity and analyte diffusion are incorporated in these plots to prove that superior performances are within experimental reach for conventional LC columns and equipment. Verification of the modified kinetic plots with experimental data points is presented.
液相色谱（LC）中温度的理论方面大多是为了阐明各种溶剂中小分子和大分子保留行为的变化而进行研究的。而温度在色谱性能中也起着重要作用这一点却鲜为人知。动力学图是一种既定工具，可根据在最大可达到的柱压下使用特定粒径所能获得的速度和效率来预测色谱性能。在本文中，将温度对流动相粘度和分析物扩散的影响纳入这些图中，以证明对于传统的液相色谱柱和设备而言，卓越的性能在实验上是可以实现的。文中还给出了用实验数据点对修正后的动力学图进行的验证。
