Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
J Chromatogr A. 2011 Nov 18;1218(46):8388-93. doi: 10.1016/j.chroma.2011.09.047. Epub 2011 Sep 22.
The kinetic optimisation of open-tubular liquid chromatography (OTLC) columns has been revisited by taking the thick-film effects for porous coatings on retention, column resistance, band broadening and mass loadability into account. Considering the most advantageous case (i.e. where the retentive layer allows for the same high internal diffusion coefficient as conventional porous particles), calculations show the need for the development of coating procedures leading to porous films filling up approximately 50-70% of the total column diameter. Furthermore, to achieve optimum kinetic performance for separations of small molecules with total analysis times of less than 8h (k'=9), total column diameters should be less than 6 μm with lengths typically greater than 0.8m for N values of 125,000-500,000 at a pressure of 400 bar. The use of elevated temperature LC (90°C) is also shown to increase the allowable total column diameter to up to 9 μm for a larger range of N values (100,000-880,000).
通过考虑多孔涂层对保留、柱阻力、带宽和质量负载能力的厚膜效应，重新研究了开管液相色谱（OTLC）柱的动力学优化。考虑到最有利的情况（即保留层允许与常规多孔颗粒相同的高内部扩散系数），计算表明需要开发涂层程序，以使多孔膜填充总柱直径的约 50-70%。此外，为了实现小分子分离的最佳动力学性能，总分析时间小于 8 小时（k'=9），总柱直径应小于 6μm，长度通常大于 0.8m，在 400 巴的压力下，N 值为 125000-500000。还表明，使用高温 LC（90°C）可将允许的总柱直径增加到 9μm，适用于更大范围的 N 值（100000-880000）。
