高效液相色谱法测定外用软膏中葡萄糖酸氯己定和对氯苯胺的含量。
HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment.
作者信息
Havlíková L, Matysová L, Nováková L, Hájková R, Solich P
机构信息
Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
出版信息
J Pharm Biomed Anal. 2007 Feb 19;43(3):1169-73. doi: 10.1016/j.jpba.2006.09.037. Epub 2006 Nov 9.
A novel fast isocratic reversed-phase HPLC method for simultaneous determination of chlorhexidine and its degradation product p-chloroaniline was developed. Zorbax SB Phenyl column (75 mm x 4.6 mm, 3.5 microm) was used for the separation. Mobile phase composed of acetonitrile and buffer solution of 0.08 M sodium phosphate monobasic containing 5 ml of triethylamine (0.5%) and adjust with 85% phosphoric acid to pH 3.0 in ratio 35:65 (v/v) pumped isocratically at flow rate 0.6 ml min(-1) was used. UV detection was performed at 239 nm, the total analysis time was about 10 min. The method is suitable for practical routine analysis of topical ointment in the quality control laboratory.
建立了一种新型快速等度反相高效液相色谱法，用于同时测定洗必泰及其降解产物对氯苯胺。采用Zorbax SB苯基柱（75 mm×4.6 mm，3.5微米）进行分离。流动相由乙腈和含5 ml三乙胺（0.5%）的0.08 M磷酸二氢钠缓冲溶液组成，用85%磷酸调至pH 3.0，比例为35:65（v/v），以0.6 ml min(-1)的流速等度泵入。在239 nm处进行紫外检测，总分析时间约为10分钟。该方法适用于质量控制实验室对局部软膏进行实际常规分析。
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