番石榴叶提取物的抗菌评估及其对氢氧化钙和氯己定混合物产生的活性氧的影响。
Antibacterial evaluation of guava leaves extract and its effect on reactive oxygen species formed by calcium hydroxide and chlorhexidine mixture.
作者信息
Chandran Karma Deepak, Karthikeyan Kittappa, Sujatha Venkatappan, Mahalaxmi Sekar
机构信息
Department of Conservative Dentistry and Endodontics, SRM Dental College, SRM Institute of Science and Technology, Ramapuram Campus, Chennai, Tamil Nadu, India.
出版信息
J Conserv Dent. 2021 Jul-Aug;24(4):389-392. doi: 10.4103/jcd.jcd_183_21. Epub 2022 Jan 13.
BACKGROUND
Reactive oxygen species (ROS) are formed when mixing calcium hydroxide (CH) with chlorhexidine (CHX).
AIM
To analyze the antibacterial efficacy of aqueous (AGL) and ethanolic (EGL) extracts of leaves against root canal bacteria such as , , and , to evaluate the antioxidant potential of the extracts and its ability to counteract the ROS.
MATERIALS AND METHODS
Three different concentrations (10%, 25%, and 35%) of AGL and EGL were prepared. The antibacterial efficacy was analyzed using the Kirby-Bauer disc diffusion method. The different concentrations of both AGL and EGL were evaluated for their antioxidant property using ultraviolet spectrometer. Further, the ability of these extracts on neutralizing the ROS formation was analyzed using mass spectrometer.
RESULTS
CH and CHX mixture exhibited maximum antibacterial activity followed by the same mixture with 35% EGL. EGL showed greater antioxidant activity than AGL extract at all concentrations.
CONCLUSIONS
The addition of natural antioxidants like guava leaves extract decreases ROS formed by CH and CHX mixture.
背景
氢氧化钙（CH）与洗必泰（CHX）混合时会产生活性氧（ROS）。
目的
分析番石榴叶水提取物（AGL）和乙醇提取物（EGL）对根管细菌（如 、 、 和 ）的抗菌效果，评估提取物的抗氧化潜力及其对抗ROS的能力。
材料与方法
制备了三种不同浓度（10%、25%和35%）的AGL和EGL。采用 Kirby-Bauer 纸片扩散法分析抗菌效果。使用紫外光谱仪评估不同浓度的AGL和EGL的抗氧化性能。此外，使用质谱仪分析这些提取物中和ROS形成的能力。
结果
CH和CHX混合物表现出最大的抗菌活性，其次是与35% EGL的相同混合物。在所有浓度下，EGL均比AGL提取物表现出更强的抗氧化活性。
结论
添加番石榴叶提取物等天然抗氧化剂可减少CH和CHX混合物产生的ROS。
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