包括Mg2+和镧系元素在内的金属离子对核糖核苷酸和RNA模型化合物裂解的影响。
Effects of metal ions, including Mg2+ and lanthanides, on the cleavage of ribonucleotides and RNA model compounds.
作者信息
Breslow R, Huang D L
机构信息
Department of Chemistry, Columbia University, New York, NY 10027.
出版信息
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4080-3. doi: 10.1073/pnas.88.10.4080.
Abstract
The cyclization/cleavage of 3',5'-uridyluridine to form 2',3'-cyclic uridylic acid is very effectively catalyzed by Eu3+, and the cyclization/cleavage of the 1-p-nitrophenyl phosphate ester of propane-1,2-diol also shows strong metal ion catalysis by Eu3+, Tb3+, and Yb3+. It also shows moderate catalysis by Mg2+, but not by Ca2+; Zn2+ and Pb2+ are also good catalysts. Various ligands activate these reactions further, and imidazole apparently acts as an additional base catalyst. Some cyclodextrin derivatives act to bind both the substrate and the metal ion but, contrary to what is reported elsewhere, there is no strong selectivity among nucleotides that can be ascribed to cyclodextrin binding.
摘要
铕（Eu3+）能非常有效地催化3',5'-尿苷基尿苷环化/裂解形成2',3'-环磷酸尿苷，1,2-丙二醇对硝基苯磷酸酯的环化/裂解也显示出铕（Eu3+）、铽（Tb3+）和镱（Yb3+）的强金属离子催化作用。镁离子（Mg2+）也显示出适度的催化作用，但钙离子（Ca2+）则无催化作用；锌离子（Zn2+）和铅离子（Pb2+）也是良好的催化剂。各种配体可进一步激活这些反应，咪唑显然起到额外的碱催化剂作用。一些环糊精衍生物能结合底物和金属离子，但与其他地方报道的情况相反，环糊精结合对核苷酸没有很强的选择性。
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