Bodle Julia F, Duffy Sean R G, Binney David M
Academic Department of Obstetrics and Gynecology, Saint James's University Hospital, Leeds University, Leeds, UK.
J Minim Invasive Gynecol. 2006 Nov-Dec;13(6):500-4. doi: 10.1016/j.jmig.2006.06.012.
To determine the effect of a 9-mm diameter carbon dioxide cryoprobe, the Endocryo, on myomas and endometrial/myometrial tissue in vitro.
Comparative laboratory study (Canadian Task Force classification II-2).
University laboratory.
Women with and without myomas, undergoing hysterectomy.
A single 5-minute freeze followed by an active thaw was applied to uterine myomas and endometrial/myometrial tissue in vitro.
Endometrial/myometrial and uterine myoma temperature change was measured continuously during the cryosurgical procedure. Depth of cell death was measured using nicotinamide adenine dinucleotide diaphorase enzyme assay. There was no significant difference in temperature change and depth of cell death between myomas and endometrial/myometrial tissue in vitro.
The Endocryo produces the same cryosurgical effect on both uterine myomas and endometrial/myometrial tissue in vitro, an important principal for future development of a clinically effective cryosurgical device for the treatment of menorrhagia in the presence of submucous myomas.
确定直径9毫米的二氧化碳冷冻探头Endocryo对体外肌瘤及子宫内膜/子宫肌层组织的作用。
对比实验室研究（加拿大工作组分类II-2）。
大学实验室。
行子宫切除术的有肌瘤和无肌瘤女性。
对体外子宫肌瘤及子宫内膜/子宫肌层组织进行单次5分钟冷冻，随后主动解冻。
在冷冻手术过程中持续测量子宫内膜/子宫肌层及子宫肌瘤的温度变化。使用烟酰胺腺嘌呤二核苷酸黄递酶酶测定法测量细胞死亡深度。体外肌瘤与子宫内膜/子宫肌层组织之间的温度变化及细胞死亡深度无显著差异。
Endocryo对体外子宫肌瘤及子宫内膜/子宫肌层组织产生相同的冷冻手术效果，这是未来开发用于治疗黏膜下肌瘤所致月经过多的临床有效冷冻手术设备的一项重要原则。
